Furthermore, 13 transcription factors were identified in the present study belonging to six different transcription factor families. Significant allelic imbalance of M1, M4 and M5 loci between growth selected and control individual were observed. Validation of eight putative SNPs using Sanger sequencing resulted in 100% true SNPs. Further, 2231 putative SNPs were identified with high confidence. In total, 289 SSRs were identified with an identification rate of 5.8%, and dinucleotide repeat motifs were observed to be the most abundant SSRs. Out of these, 4171 had significant blast hit against NCBI nr database, and 2130 transcripts were successfully annotated. In total, 1.2 million reads were generated, de novo assembly and clustering resulted in 4171 transcripts. Here, we have characterized the liver and muscle transcriptomes of rohu using Roche 454 GS-FLX next generation sequencing platform. Transcriptome resource for this species is scanty. A selective breeding program for harvest body weight has resulted in an average genetic gain of 17% per generation. Labeo rohita, one of the Indian major carps, is the most popular culture species in Indian subcontinent due to its consumer preference and delicacy.
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January 2023
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